Report: Dietary-protein sources modulate host susceptibility to Clostridioides difficile infection through the gut microbiota. Image Credit: valzan / Shutterstock

Soy protein diet increases susceptibility to Clostridium difficile infection

According to a recent study published in the journal cell reportsA diet rich in soy protein increases the human Clostridium difficile Vulnerability by increasing levels of gut amino acids (AAs) and promoting growth Lactobacillus. Lactobacillusin turn, digest the soy protein to produce amino acids, which again facilitates Jim Saab, And therefore, Clostridium difficile infection (CDI).

a report: Dietary protein sources modulate host susceptibility to Clostridium difficile infection through the gut microbiota. Image Credit: valzan / Shutterstock


bacteria Jim Saab It causes inflammation of the large intestine (colon). Jim Saab he is Gram-positive, forming spores obligate anaerobes that are ubiquitous in the large intestine and cause nosocomial infections

A variety of symptoms can be experienced, from mild diarrhea to severe colon damage. In addition, the use of antibiotics is often associated with the development of Jim Saab illness. This disease primarily affects elderly patients who are admitted to hospitals or long-term care facilities.

It is noticeable that Jim Saab Colonization resistance decreases after antibiotic treatment, leading to dysbacteriosis of the gut microbiota. Competing with the gut microbiota Jim Saab of nutrients and also produces certain metabolites that inhibit Jim Saab colonization.

Human gut health and susceptibility to CDI are significantly influenced by dietary factors. To relieve gut inflammation, indigestible carbohydrates help boost it Jim Saab Clearance by stimulating short-chain fatty acid (SCFA) production by gut microbiota. Many nutrient-rich diets, for example, those high in fat, zinc, and protein, exacerbate CDI by altering the composition of the gut microbiota.

However, it is not known how specific food components interact with the gut microbiota to affect CDI. There is no information available regarding specific nutritional factors that affect the gut microbiota in terms of its composition and metabolism.

the study

The current study investigated how certain food components interact with the gut microbiota and how this interaction might alter the severity of CDI.

This study was performed in pathogen-free C57BL/6 WT female mice (5 weeks old). Fecal samples were collected from individual mice after cefoperazone treatment and just before infection to analyze gut microbiota constitution and determine fecal AA levels.

The diet of these animals was modified with two main sources of protein – soy (SD) and casein (PD). The antibiotic-treated mice were divided into two groups – those fed a normal diet (RD) and those fed a regular diet (PD). The researchers then used CE-TOFMS to compare gut metabolites from mice fed RD and PD. The gut microbiota was then studied in response to RD and SD.

16S rRNA genes were studied in the mouse faecal microbiota. The researchers used whole-genome sequencing to detect cell envelope protease (CEP) in an isolated state lactobacillus mouse. After that, prtP is minus L. murinus (Lm prtP) strains were generated. Then the researchers tested whether Lm prtP could grow in modified trace medium, especially soybean.

the findings

It was found that the host’s diet influences their susceptibility to CDI because RD exacerbates CDI outcomes by enhancing Jim Saab growth in the digestive system. In the gut, soy protein meals raise and boost AA levels Jim Saab spread.

According to the principal coordinates analysis, fecal metabolites differed significantly between mice fed either RD or PD. There was a significant difference in fecal levels between RD-fed mice and PD-fed mice with respect to most AAs and their derivatives between the two groups.

c difficult Growth is promoted by soy protein meals by increasing the concentration of amino acids in the intestine. Soybean protein promotes growth Lactobacillus Species that produce AAs. According to 16S rRNA gene analysis of rat fecal microbiota, Lactobacillus Sex was more prevalent in antibiotic-treated mice fed RD and SD.

Soy protein is preferred over casein L. murinus To increase AA levels. PrtP is an important enzyme in for merinos which increases and enhances AA levels Jim Saab growth. AAs are provided by for merinos in a PrtP-dependent manner and is instrumental in promoting the growth of Clostridium difficile.

Collectively, the results indicated that dietary soy protein enhances Lactobacillus growth and increased levels of AA in the gut during antibiotic-induced dysbiosis, allowing Jim Saab colonization and growth. It has also been observed that in the presence of soy protein, L. murinus It grew and produced more AAs than there was casein. In addition, the PrtP protease in the extracellular cell wall contributed to the increased levels of AA. L. murinusisolated from the faces of mice, produced AAs using soy protein instead of casein.


The cross-reactivity between diet and gut microbiota may influence susceptibility to CDI. For example, a high-protein diet or treatment with AA fermenting bacteria after antibiotic treatment may reduce intestinal AA levels and effectively prevent CDI.

Given that diet and gut microbes interact in order to control Jim Saab growth, this research provides further evidence that specific gut microbiota and microbial-derived metabolites influence host susceptibility to intestinal pathogens.

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